@article {14,
	title = {Extreme diversity of tropical parasitoid wasps exposed by iterative integration of natural history, DNA barcoding, morphology, and collections},
	journal = {Proceedings of the National Academy of Sciences of the United States of America},
	volume = {105},
	number = {34},
	year = {2008},
	note = {Times Cited: 6},
	pages = {12359-12364},
	abstract = {We DNA barcoded 2,597 parasitoid wasps belonging to 6 microgastrine braconid genera reared from parapatric tropical dry forest, cloud forest, and rain forest in Area de Conservacion Guanacaste (ACG) in northwestern Costa Rica and combined these data with records of caterpillar hosts and morphological analyses. We asked whether barcoding and morphology discover the same provisional species and whether the biological entities revealed by our analysis are congruent with wasp host specificity. Morphological analysis revealed 171 provisional species, but barcoding exposed an additional 142 provisional species; 95\% of the total is likely to be undescribed. These 313 provisional species are extraordinarily host specific; more than 90\% attack only 1 or 2 species of caterpillars out of more than 3,500 species. sampled. The most extreme case of overlooked diversity is the morphospecies Apanteles leucostigmus. This minute black wasp with a distinctive white wing stigma was thought to parasitize 32 species of ACG hesperiid caterpillars, but barcoding revealed 36 provisional species, each attacking one or a very few closely related species of caterpillars. When host records and/or within-ACG distributions suggested that DNA barcoding had missed a species-pair, or when provisional species were separated only by slight differences in their barcodes, we examined nuclear sequences to test hypotheses of presumptive species boundaries and to further probe host specificity. Our iterative process of combining morphological analysis, ecology, and DNA barcoding and reiteratively using specimens maintained in permanent collections has resulted in a much more fine-scaled understanding of parasitoid diversity and host specificity than any one of these elements could have produced on its own.},
	isbn = {0027-8424},
	url = {<Go to ISI>://WOS:000258905700048 },
	author = {Smith, M. A. and Rodriguez, J. J. and Whitfield, J. B. and Deans, A. R. and Janzen, D. H. and Hallwachs, W. and Hebert, P. D. N.}
}
@article {8,
	title = {Integration of DNA barcoding into an ongoing inventory of complex tropical biodiversity},
	journal = {Molecular Ecology Resources},
	volume = {9},
	year = {2009},
	note = {Times Cited: 1},
	pages = {1-26},
	abstract = {Inventory of the caterpillars, their food plants and parasitoids began in 1978 for today{\textquoteright}s Area de Conservacion Guanacaste (ACG), in northwestern Costa Rica. This complex mosaic of 120 000 ha of conserved and regenerating dry, cloud and rain forest over 0-2000 m elevation contains at least 10 000 species of non-leaf-mining caterpillars used by more than 5000 species of parasitoids. Several hundred thousand specimens of ACG-reared adult Lepidoptera and parasitoids have been intensively and extensively studied morphologically by many taxonomists, including most of the co-authors. DNA barcoding - the use of a standardized short mitochondrial DNA sequence to identify specimens and flush out undisclosed species - was added to the taxonomic identification process in 2003. Barcoding has been found to be extremely accurate during the identification of about 100 000 specimens of about 3500 morphologically defined species of adult moths, butterflies, tachinid flies, and parasitoid wasps. Less than 1\% of the species have such similar barcodes that a molecularly based taxonomic identification is impossible. No specimen with a full barcode was misidentified when its barcode was compared with the barcode library. Also as expected from early trials, barcoding a series from all morphologically defined species, and correlating the morphological, ecological and barcode traits, has revealed many hundreds of overlooked presumptive species. Many but not all of these cryptic species can now be distinguished by subtle morphological and/or ecological traits previously ascribed to {\textquoteright}variation{\textquoteright} or thought to be insignificant for species-level recognition. Adding DNA barcoding to the inventory has substantially improved the quality and depth of the inventory, and greatly multiplied the number of situations requiring further taxonomic work for resolution.},
	isbn = {1755-098X},
	url = {<Go to ISI>://WOS:000265227700001 },
	author = {Janzen, D. H. and Hallwachs, W. and Blandin, P. and Burns, J. M. and Cadiou, J. M. and Chacon, I. and Dapkey, T. and Deans, A. R. and Epstein, M. E. and Espinoza, B. and Franclemont, J. G. and Haber, W. A. and Hajibabaei, M. and Hall, J. P. W. and Hebert, P. D. N. and Gauld, I. D. and Harvey, D. J. and Hausmann, A. and Kitching, I. J. and Lafontaine, D. and Landry, J. F. and Lemaire, C. and Miller, J. Y. and Miller, J. S. and Miller, L. and Miller, S. E. and Montero, J. and Munroe, E. and Green, S. R. and Ratnasingham, S. and Rawlins, J. E. and Robbins, R. K. and Rodriguez, J. J. and Rougerie, R. and Sharkey, M. J. and Smith, M. A. and Solis, M. A. and Sullivan, J. B. and Thiaucourt, P. and Wahl, D. B. and Weller, S. J. and Whitfield, J. B. and Willmott, K. R. and Wood, D. M. and Woodley, N. E. and Wilson, J. J.}
}
